Effects of mutations in the substrate-binding domain of poly[(R)-3-hydroxybutyrate] (PHB) depolymerase from Ralstonia pickettii T1 on PHB degradation
Article Abstract:
The interaction between denatured poly[(R)-3-hydroxybutyrte] (dPHB) depolymerase and the dPHB surface is investigated by a combination of PCR random mutagenesis targeted only to the substrate-binding domain (SBD) region and an in vivo screening system to identify the amino acid residues relating to dPHB adsorption. The kinetic analysis of the dPHB degradation suggested that lowering the affinity of the SBD towards dPHB causes a decrease in the dPHB degradation rate without the loss of its hydrolytic activity for the polymer chain.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Coexpression of genetically engineered 3-Ketoacyl-ACP synthase III (fabH) and Polyhydroxyalkanoate synthase (phaC) genes leads to short-chain-length-medium-chain-length Polyhydroxyalkanoate copolymer production from glucose in Escherichia coli JM109
Article Abstract:
Polyhydroxyalkanoates (PHAs) are polyesters of 3-hydroxy alkanoic acids that are produced as intracellular granules by different bacteria. A study was conducted to prove that short0chain-length (SCL) medium-chain-length (MCL) PHAs can be made from glucose in recombinant E. coli.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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